Sztein J, Sweet H, Farley J, Mobraaten L
The Jackson Laboratory, Bar Harbor, Maine 04609, USA.
Biol Reprod. 1998 Apr;58(4):1071-4. doi: 10.1095/biolreprod58.4.1071.
Mouse half ovaries were cryopreserved and orthotopically transplanted into ovariectomized recipients genetically identical to ovary donors except for the coat color gene. Fertility was reestablished in 57% of the female recipients, which became pregnant in an average of 40 days after transplantation of frozen-thawed half ovaries. These experiments demonstrate that ovary cryopreservation can be a very useful option for banking mouse germplasm, or managing subfertile animal colonies, when embryo or sperm freezing cannot be used or is not cost effective.
将小鼠半卵巢进行冷冻保存，并原位移植到除毛色基因外与卵巢供体基因相同的去卵巢受体中。57%的雌性受体恢复了生育能力，在移植冻融半卵巢后平均40天怀孕。这些实验表明，当无法使用胚胎或精子冷冻或其成本效益不高时，卵巢冷冻保存对于保存小鼠种质或管理亚生育动物群体可能是一个非常有用的选择。
