Liu L, Wood G A, Morikawa L, Ayearst R, Fleming C, McKerlie C
Physiology and Experimental Medicine, The Hospital For Sick Children, 555 University Ave, Toronto, Ontario, Canada M5G 1X8.
Hum Reprod. 2008 Jan;23(1):122-8. doi: 10.1093/humrep/dem348. Epub 2007 Nov 9.
Successful thawing and orthotopic transplantation of ovarian tissue has produced live offspring in mice, but until now has only been successful for very young ovary donors.
Whole and half ovaries from adult C3H/HeNCrlBR (C3H) and whole ovaries from B6129SF1/J were frozen-thawed and then grafted orthotopically into B6C3F1/CrlBR (B6C3F1) and B6129SF1/J recipients, respectively. In bilateral transplant groups (bilateral), recipients underwent a bilateral ovariectomy, followed by orthotopic grafting. In unilateral groups recipients either underwent bilateral ovariectomy followed by unilateral grafting (unilateral(ovx)) or had only one ovary removed and replaced with a graft (unilateral) along with complete transection of the remaining oviduct.
Ovary size and number of follicles decreased dramatically in grafted compared with control groups, but the loss in the unilateral(ovx) group was significantly less than in the unilateral group. Similar numbers of litters and litter size were obtained in bilateral and unilateral grafts of fresh ovary. However, a much lower number of litters and litter size were derived from unilateral grafts than from unilateral(ovx) grafts of frozen ovary.
Normal fertility can be restored by orthotopic grafting of fresh or frozen adult mouse ovaries and no significant difference between fresh and frozen ovaries was found. Grafting of half ovaries does not alter the overall fertility rate. Unilateral(ovx) grafting is an efficient procedure to produce live pups and removes the negative effect of recipient native ovaries on post-grafting fertility.
卵巢组织的成功解冻和原位移植已在小鼠中产生了活体后代，但迄今为止仅在非常年轻的卵巢供体中取得成功。
将成年C3H/HeNCrlBR（C3H）的整个卵巢和半侧卵巢以及B6129SF1/J的整个卵巢进行冻融，然后分别原位移植到B6C3F1/CrlBR（B6C3F1）和B6129SF1/J受体中。在双侧移植组（双侧）中，受体接受双侧卵巢切除术，然后进行原位移植。在单侧组中，受体要么接受双侧卵巢切除术，然后进行单侧移植（单侧（去卵巢）），要么仅切除一个卵巢并用移植体替换（单侧），同时将剩余输卵管完全横断。
与对照组相比，移植后的卵巢大小和卵泡数量显著减少，但单侧（去卵巢）组的损失明显小于单侧组。新鲜卵巢的双侧和单侧移植获得的窝数和窝仔数相似。然而，冷冻卵巢的单侧移植产生的窝数和窝仔数远低于单侧（去卵巢）移植。
新鲜或冷冻的成年小鼠卵巢原位移植可恢复正常生育能力，新鲜和冷冻卵巢之间未发现显著差异。半侧卵巢移植不会改变总体生育率。单侧（去卵巢）移植是产生活仔的有效方法，可消除受体天然卵巢对移植后生育能力的负面影响。
