Hohlweg W, Dauber U
Endokrinologie. 1976 Jun;67(2):240-3.
Infantile female rats were subcutaneously injected with 2.5 mg testosterone within two days. The majority of the rats responded to this treatment with opening of the vagina, and about 50% developed an estrus. However, corpora lutea were not formed. Equally treated animals received additionally 0.2 i.u. HCG -- a dose which is inffective when given alone -- distributed on the second and third day of experiment. After this treatment an increased number of rats reacted with estrus and the development of corpora lutea. In infantile female rats, follicles stimulated by testosterone were also transformed into corpora lutea by an injection of 0.025 mg clomiphene citrate.
在出生两天内的雌性幼鼠皮下注射2.5毫克睾酮。大多数大鼠对此治疗的反应是阴道开口，约50%出现发情。然而，未形成黄体。同样接受上述处理的动物在实验的第二天和第三天额外注射0.2国际单位人绒毛膜促性腺激素（单独使用该剂量无效）。经过此处理后，发情并形成黄体的大鼠数量增加。在出生两天内的雌性幼鼠中，注射0.025毫克枸橼酸氯米芬也能使受睾酮刺激的卵泡转化为黄体。
