通过铱（IV）氧化对7,8 - 二氢 - 8 - 氧代鸟嘌呤和7,8 - 二氢 - 8 - 氧代腺嘌呤进行凝胶电泳检测。
Gel electrophoretic detection of 7,8-dihydro-8-oxoguanine and 7, 8-dihydro-8-oxoadenine via oxidation by Ir (IV).
作者信息
Muller J G, Duarte V, Hickerson R P, Burrows C J
机构信息
Department of Chemistry, University of Utah, 315 South 1400 East, RM Dock, Salt Lake City, UT 84112-0850, USA.
出版信息
Nucleic Acids Res. 1998 May 1;26(9):2247-9. doi: 10.1093/nar/26.9.2247.
Abstract
Two gel electrophoretic methods are described for detection of 7, 8-dihydro-8-oxoguanine and 7,8-dihydro-8-oxoadenine based on their further oxidation with one-electron oxidants including IrCl62-and IrBr62-. The products of nucleobase oxidation lead to enhanced piperidine-sensitive cleavage and to highly visible stop points in a primer extension assay. 8-oxoG and 8-oxoA lesions may be distinguished by the latter's inability to be oxidized by IrBr62-compared to IrCl62-Comparison is also made to oxidation by MnO4-.
摘要
本文描述了两种凝胶电泳方法，用于检测7,8 - 二氢 - 8 - 氧代鸟嘌呤和7,8 - 二氢 - 8 - 氧代腺嘌呤，该方法基于它们与单电子氧化剂（包括IrCl62-和IrBr62-）的进一步氧化反应。在引物延伸分析中，核碱基氧化产物会导致哌啶敏感性切割增强，并产生高可见度的终止点。与IrCl62-相比，8 - 氧代鸟嘌呤（8-oxoG）和8 - 氧代腺嘌呤（8-oxoA）损伤可以通过后者不能被IrBr62-氧化来区分。同时还与MnO4-氧化法进行了比较。
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