Asano Yoshiya, Nakamura Satoshi, Ishida Sachiko, Azuma Katsu, Shinozawa Takao
J Exp Biol. 1998 May;201 (Pt 9):1263-71. doi: 10.1242/jeb.201.9.1263.
The presence of rhodopsin-like proteins in the eyes and auricles of the freshwater planarian Dugesia japonica was confirmed using anti-frog-rhodopsin rabbit IgG. The apparent relative molecular masses of these proteins were 65x10(3) and 62x10(3), and positive reactions to IgG were localized to the microvilli of the photoreceptor cells in the eyes and to the sensory cilia, rootlets and microvilli in the auricles. Eye- or head-excised planarians showed no negative phototaxis, whereas intact or auricle-excised planarians did. During regeneration in head-excised planarians, the appearance of rhodopsin-like proteins in the regenerating eyes corresponded to the recovery of negative phototaxis behaviour. Head or auricle excision enhanced asexual fission under continuous illumination. However, eye excision had no such effect. These results suggest that the rhodopsin-like proteins in the eyes work as photoreceptors for negative phototaxis behaviour and that, in the auricles, they are involved in asexual fission originating from the circadian rhythm.
使用抗蛙视紫红质兔IgG证实了淡水涡虫日本三角涡虫的眼睛和触角中存在视紫红质样蛋白。这些蛋白质的表观相对分子质量分别为65×10³和62×10³，IgG的阳性反应定位于眼睛中光感受器细胞的微绒毛以及触角中的感觉纤毛、小根和微绒毛。切除眼睛或头部的涡虫没有负趋光性，而完整的或切除触角的涡虫有。在切除头部的涡虫再生过程中，再生眼睛中视紫红质样蛋白的出现与负趋光性行为的恢复相对应。切除头部或触角会增强在持续光照下的无性分裂。然而，切除眼睛没有这种效果。这些结果表明，眼睛中的视紫红质样蛋白作为负趋光性行为的光感受器起作用，并且在触角中，它们参与源自昼夜节律的无性分裂。
