Suzuki M, Kadir A, Hayashi N, Takamoto M
Department of Otolaryngology, Hiroshima University, School of Medicine, Japan.
J Vestib Res. 1998 Mar-Apr;8(2):169-73.
Effect of thermal stimulus on the vestibular receptor was studied using the isolated frog semicircular canal. The posterior (PSC) and lateral semicircular canals (LSC) were placed in the horizontal plane in frog Ringer's solution. The ampullary nerve was sucked into a glass suction electrode to record compound potentials. The steel thermal probe was positioned next to the ampullary surface to give thermal stimuli. When the PSC ampulla was cooled, the spontaneous discharge markedly increased. When the PSC ampulla was warmed, the discharge decreased. When the LSC ampulla was cooled, the discharge increased in the same manner as in the PSC. Also, warming of the LSC decreased the discharge in the same manner as in the PSC. The cupula was removed from the crista in order to eliminate the effect of volume change of the endolymph. The results were comparable to those with intact cupula in both the PSC and LSC. Cooling increased, while warming decreased the discharge. These responses are possibly due to a mechanism other than mechanical volume change, because the PSC and LSC yielded the same type of responses. Direct temperature reaction of the vestibular hair cell was suggested.
使用离体青蛙半规管研究热刺激对前庭感受器的影响。将后半规管（PSC）和外侧半规管（LSC）置于盛有青蛙任氏液的水平平面中。将壶腹神经吸入玻璃吸引电极以记录复合电位。将钢制热探头置于壶腹表面旁边以给予热刺激。当PSC壶腹冷却时，自发放电明显增加。当PSC壶腹升温时，放电减少。当LSC壶腹冷却时，放电以与PSC相同的方式增加。同样，LSC升温也以与PSC相同的方式减少放电。为消除内淋巴体积变化的影响，将终帽从嵴上移除。结果在PSC和LSC中均与具有完整终帽时相当。冷却使放电增加，而升温使放电减少。这些反应可能归因于除机械体积变化之外的其他机制，因为PSC和LSC产生相同类型的反应。提示存在前庭毛细胞的直接温度反应。
