Smith C J, Morrow J D, Roberts L J, Marnett L J
A.B. Hancock, Jr. Memorial Laboratory of Cancer Research, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
Adv Exp Med Biol. 1997;400A:99-106. doi: 10.1007/978-1-4615-5325-0_14.
Prostaglandin endoperoxide synthase (PGH synthase) is responsible for converting arachdonic acid to PGH2, the common precursor of prostaglandins. It has been shown previously that phorbol ester-induced differentiation of human promonocytic leukemia cell lines is accompanied by induction of PGH synthase enzyme and enhanced capacity to produce prostaglandins. However, the identity of the isoform of PGH synthase, i.e., PGH synthase-1 or -2, that is induced under these conditions has not been established. Northern and Western analyses revealed a dramatic increase in levels of PGH synthase-1 mRNA and protein levels within 24 hr after treatment of THP-1 cells with phorbol ester. No significant increase in PGH synthase-2 mRNA or protein was observed. The increases in PGH synthase-1 were accompanied by an enhanced capacity of the cells to produce PGE2. The current findings indicate that expression of PGH synthase-1 is greatly enhanced in a promonocytic cell line by treatment with an agent that induces differentiation.
前列腺素内过氧化物合酶（PGH合酶）负责将花生四烯酸转化为PGH2，而PGH2是前列腺素的共同前体。先前已经表明，佛波酯诱导的人原单核细胞白血病细胞系分化伴随着PGH合酶的诱导以及产生前列腺素能力的增强。然而，在这些条件下被诱导的PGH合酶同工型，即PGH合酶-1或-2的身份尚未确定。Northern和Western分析显示，用佛波酯处理THP-1细胞后24小时内，PGH合酶-1 mRNA和蛋白质水平显著增加。未观察到PGH合酶-2 mRNA或蛋白质有显著增加。PGH合酶-1的增加伴随着细胞产生PGE2能力的增强。目前的研究结果表明，用诱导分化的试剂处理可使原单核细胞系中PGH合酶-1的表达大大增强。
