Cotton F, Adler M, Dumon J, Boeynaems J M, Gulbis B
Department of Clinical Chemistry, Hôpital Erasme Université Libre de Bruxelles, Belgium.
Ann Clin Biochem. 1998 Mar;35 ( Pt 2):268-73. doi: 10.1177/000456329803500211.
Carbohydrate-deficient transferrin (CDT) is known to be increased in alcohol abuse. Several methods were developed for its measurement (e.g. isoelectric focusing with Western blotting or immunofixation, anion-exchange chromatography followed by immunoassays). We describe a greatly simplified isoelectric focusing technique which does not require immunofixation. CDT results obtained with this method were compared to other biological markers of alcohol abuse, i.e. mean corpuscular volume (MCV), aspartate aminotransferase (ASAT) and gamma-glutamyl-transferase (GGT), in 55 patients distributed in three groups (i.e. healthy control subjects, control patients suffering from various hepato-gastrointestinal diseases and alcohol abusing patients). Sensitivity and specificity were 33-89%, 61-57%, 89-49% and 83-100% for MCV, ASAT, GGT and CDT, respectively. We conclude that our method is highly suitable for routine clinical use.
已知酒精滥用会导致转铁蛋白糖基化缺乏（CDT）升高。目前已开发出多种测量方法（如采用蛋白质印迹法或免疫固定法的等电聚焦、阴离子交换色谱法结合免疫分析）。我们描述了一种无需免疫固定的大大简化的等电聚焦技术。将用该方法获得的CDT结果与酒精滥用的其他生物学标志物，即平均红细胞体积（MCV）、天冬氨酸转氨酶（ASAT）和γ-谷氨酰转移酶（GGT），在分为三组的55例患者中进行比较（即健康对照受试者、患有各种肝胃肠疾病的对照患者和酒精滥用患者）。MCV、ASAT、GGT和CDT的敏感性和特异性分别为33 - 89%、61 - 57%、89 - 49%和83 - 100%。我们得出结论，我们的方法非常适合常规临床应用。
