Nassar A, Mahony M, Blackmore P, Morshedi M, Ozgur K, Oehninger S
The Jones Institute for Women's Health, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk 23507, USA.
Fertil Steril. 1998 Apr;69(4):748-54. doi: 10.1016/s0015-0282(98)00013-2.
To investigate the effects of the phosphodiesterase inhibitor pentoxifylline on hyperactivated motility and acrosome reaction in human sperm and to determine whether its stimulatory effects occur via increased intracellular calcium levels.
Prospective study.
Academic tertiary care facility.
PARTICIPANT(S): Healthy male donors.
INTERVENTION(S): The effects of pentoxifylline on hyperactivated motility, acrosome reaction, and intracellular calcium were studied and compared with the effects of progesterone. Thapsigargin, a known mobilizer of intracellular calcium, also was used as positive control.
MAIN OUTCOME MEASURE(S): Hyperactivated motility was assessed by computer-assisted sperm motion analysis using the HTM-IVOS, acrosome reaction was evaluated with the fluorescent probe fluorescein isothiocyanate-labeled Pisum sativum agglutinin, and intracellular calcium was determined by fura-2 using spectrofluorometry.
RESULT(S): Pentoxifylline significantly increased both hyperactivated motility and acrosome reaction. Enhancement of hyperactivated motility by pentoxifylline in the capacitation medium persisted for up to 5 hours after pentoxifylline was washed from the medium. It also enhanced the percentage of acrosome-reacted spermatozoa after 4 hours of incubation. These effects occurred in the presence of a marginally significant decrease in intracellular calcium.
CONCLUSION(S): Pentoxifylline stimulates hyperactivated motility and acrosome reaction in spermatozoa from fertile men. Its stimulatory effects occur through mechanism(s) other than increase in intracellular calcium.
研究磷酸二酯酶抑制剂己酮可可碱对人类精子超活化运动及顶体反应的影响，并确定其刺激作用是否通过增加细胞内钙水平而发生。
前瞻性研究。
学术性三级医疗保健机构。
健康男性捐赠者。
研究己酮可可碱对超活化运动、顶体反应和细胞内钙的影响，并与孕酮的作用进行比较。毒胡萝卜素，一种已知的细胞内钙动员剂，也用作阳性对照。
使用HTM-IVOS通过计算机辅助精子运动分析评估超活化运动，用荧光探针异硫氰酸荧光素标记的豌豆凝集素评估顶体反应，用fura-2通过荧光分光光度法测定细胞内钙。
己酮可可碱显著增加超活化运动和顶体反应。在从培养基中洗去己酮可可碱后，己酮可可碱在获能培养基中对超活化运动的增强作用持续长达5小时。孵育4小时后，它还提高了顶体反应精子的百分比。这些作用在细胞内钙略有显著降低的情况下发生。
己酮可可碱刺激有生育能力男性精子的超活化运动和顶体反应。其刺激作用通过细胞内钙增加以外的机制发生。
