de Yebra L, Ballescá J L, Vanrell J A, Corzett M, Balhorn R, Oliva R
Human Genome Research Group, Faculty of Medicine, University of Barcelona, Spain.
Fertil Steril. 1998 Apr;69(4):755-9. doi: 10.1016/s0015-0282(98)00012-0.
To determine whether the reduction in the protamine P2 content (increased P1/P2 ratio) reported in some infertile patients could result from incomplete processing of protamine P2 precursors.
Analysis of samples with a marked reduction in the protamine P2 content using polyacrylamide gel electrophoresis and subsequent detection of protamine P2 precursors through Western blot analysis.
University departments and laboratories.
PATIENT(S): One hundred eighty-four men undergoing an evaluation for infertility.
MAIN OUTCOME MEASURE(S): Comparative Western blot analysis of nuclear sperm proteins using specific antibodies to protamine P1 and protamine P2.
RESULT(S): After selection of the samples with a marked reduction of the protamine P2 content and subsequent analysis by Western blot, a small proportion of putative P2 precursors was detected in most samples, whereas a significant increase was detected in two of them.
CONCLUSION(S): In some infertile men, a reduction in the protamine P2 content relative to protamine P1 (increased P1/P2 ratio) is detected concomitant with an increase in the amount of putative P2 precursors. This could represent the first report of incomplete processing of a nuclear sperm protein in humans.
确定一些不育患者中报道的鱼精蛋白P2含量降低（P1/P2比值升高）是否可能是由于鱼精蛋白P2前体加工不完全所致。
使用聚丙烯酰胺凝胶电泳分析鱼精蛋白P2含量显著降低的样本，并通过蛋白质印迹分析随后检测鱼精蛋白P2前体。
大学院系和实验室。
184名接受不育评估的男性。
使用针对鱼精蛋白P1和鱼精蛋白P2的特异性抗体对精子核蛋白进行蛋白质印迹比较分析。
在选择鱼精蛋白P2含量显著降低的样本并随后进行蛋白质印迹分析后，大多数样本中检测到一小部分假定的P2前体，而其中两个样本中检测到显著增加。
在一些不育男性中，检测到相对于鱼精蛋白P1的鱼精蛋白P2含量降低（P1/P2比值升高），同时假定的P2前体数量增加。这可能是人类精子核蛋白加工不完全的首次报道。
