Takayama Tomoko, Katayose Haruo, Yanagida Kaoru, Sato Akira
Department of Obstetrics and Gynecology, School of Medicine Fukushima Medical University Fukushima Japan.
Center for Infertility and IVF, Internal University of Health and Welfare Hospital Internal University of Health and Welfare 537-3 Iguchi 329-2763 Nasushiobara Tochigi Japan.
Reprod Med Biol. 2009 Apr 8;8(2):63-69. doi: 10.1007/s12522-009-0010-4. eCollection 2009 Jun.
To assess the influence of structural differences in sperm nuclei on embryo development in intracytoplasmic sperm injection (ICSI).
Semen obtained from forty-four infertile patients who underwent ICSI was examined. In assessing blastocyst development, only those patients who had successfully obtained over five fertilized eggs were included to exclude any oocyte factors ( = 22). Spermatozoa were assessed using excitation fluorescence flow cytometry with acridine orange and the sperm chromatin dispersion (SCD) test.
There was a significant positive correlation between the COMP values obtained from flow cytometry and blastocyst formation. ( = 0.477, = 0.025). There was a significant negative correlation between the SCD values representing DNA fragmentation and blastocyst formation. ( = 0.796, < 0.001). COMP values and SCD values were independent parameters to assess sperm nuclear quality regarding embryo development in vitro ( = 0.224, = 0.080).
Results suggest that injection of spermatozoa with fewer disulfide bonds and less nuclear DNA fragmentation could achieve better blastocyst formation in human ICSI. Assessment of sperm chromatin should help to predict embryo development after ICSI.
评估胞浆内单精子注射（ICSI）中精子细胞核结构差异对胚胎发育的影响。
对44例行ICSI的不育患者获取的精液进行检查。在评估囊胚发育时，仅纳入成功获得5个以上受精卵的患者以排除任何卵母细胞因素（n = 22）。使用吖啶橙激发荧光流式细胞术和精子染色质扩散（SCD）试验对精子进行评估。
流式细胞术获得的COMP值与囊胚形成之间存在显著正相关（r = 0.477，P = 0.025）。代表DNA片段化的SCD值与囊胚形成之间存在显著负相关（r = 0.796，P < 0.001）。COMP值和SCD值是评估体外胚胎发育精子核质量的独立参数（r = 0.224，P = 0.080）。
结果表明，注射二硫键较少且核DNA片段化较少的精子可在人类ICSI中实现更好的囊胚形成。评估精子染色质应有助于预测ICSI后的胚胎发育。
