Bider D, Livshits A, Yonish M, Yemini Z, Mashiach S, Dor J
Department of Obstetrics and Gynecology, Sackler Faculty of Medicine, Tel Aviv University, Israel.
Hum Reprod. 1997 Feb;12(2):317-20. doi: 10.1093/humrep/12.2.317.
The study was conducted to examine the efficacy of the zona drilling technique for promotion of successful implantation and pregnancy by assisting embryo hatching in women of advanced age undergoing treatment by in-vitro fertilization. A total of 839 embryos from 211 patients aged >38 years underwent assisted hatching during 312 cycles of therapy. The outcome of this micromanipulation procedure was compared to 540 non-hatched pre-embryos transferred to 174 patients during 274 cycles of therapy. Assisted hatching was performed on four- to eight-cell stage embryos using the zona drilling technique. In the assisted hatching group, 839 micromanipulated embryos were replaced (two to four embryos per patient) compared to 540 embryos transferred in the control group. Despite the fact that the pregnancy rate was not statistically different between the groups (8.9% in the assisted hatching group versus 5.1% in the controls) a trend towards an increase was noted in the assisted hatching group. The implantation rate was 3.75 and 3.55% per patient respectively, and there was no significant difference in abortion rate between the groups. The delivery rate was 3.8 and 3.4% per cycle respectively. The results of this study demonstrated that assisted hatching by zona drilling in a selected group of patients aged >38 years does not increase the take-home baby rate after in-vitro fertilization treatment.
本研究旨在通过辅助胚胎孵化，检验透明带钻孔技术对高龄体外受精女性成功着床和妊娠的促进效果。在312个治疗周期中，对211例年龄大于38岁患者的839枚胚胎进行了辅助孵化。将这种显微操作程序的结果与在274个治疗周期中移植给174例患者的540枚未孵化的前胚胎进行比较。使用透明带钻孔技术对四至八细胞期胚胎进行辅助孵化。在辅助孵化组，共移植了839枚经显微操作的胚胎（每位患者移植两至四枚胚胎），而对照组移植了540枚胚胎。尽管两组的妊娠率无统计学差异（辅助孵化组为8.9%，对照组为5.1%），但辅助孵化组有上升趋势。每位患者的着床率分别为3.75%和3.55%，两组的流产率无显著差异。每个周期的分娩率分别为3.8%和3.4%。本研究结果表明，在选定的年龄大于38岁的患者组中，通过透明带钻孔进行辅助孵化并不能提高体外受精治疗后的活产率。
