Youn B S, Zhang S M, Lee E K, Park D H, Broxmeyer H E, Murphy P M, Locati M, Pease J E, Kim K K, Antol K, Kwon B S
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46202, USA.
J Immunol. 1997 Dec 1;159(11):5201-5.
A new member of human beta-chemokine cDNA was isolated and named leukotactin-1 (Lkn-1). Lkn-1, along with murine macrophage inflammatory protein-related protein-1 and -2, defines a subgroup of beta-chemokines based on two conserved cysteines in addition to the four others conserved in all beta-chemokines. The putative mature Lkn-1 is composed of 92 amino acids with a calculated m.w. of 10,162. The Lkn-1 gene was mapped to human chromosome 17, region q12. Recombinant Lkn-1 was a potent chemoattractant for neutrophils, monocytes, and lymphocytes and induced calcium flux in these cells. Lkn-1 specifically induced calcium flux in CCR1- and CCR3-expressing HOS cell lines. Lkn-1 suppressed colony formation by human granulocyte-macrophage, erythroid, and multipotential progenitor cells stimulated by combinations of growth factors. Hence, we have isolated and characterized a human C6 beta-chemokine that is a potent agonist at CCR1 and CCR3 and shows broad biologic activities, including leukocyte chemoattraction.
一种新的人类β-趋化因子cDNA成员被分离出来并命名为白细胞趋化素-1（Lkn-1）。Lkn-1与小鼠巨噬细胞炎性蛋白相关蛋白-1和-2一起，除了在所有β-趋化因子中都保守的另外四个半胱氨酸外，还基于两个保守的半胱氨酸定义了β-趋化因子的一个亚组。推测的成熟Lkn-1由92个氨基酸组成，计算分子量为10,162。Lkn-1基因被定位到人类染色体17的q12区域。重组Lkn-1对中性粒细胞、单核细胞和淋巴细胞是一种有效的趋化剂，并能在这些细胞中诱导钙流。Lkn-1在表达CCR1和CCR3的HOS细胞系中特异性地诱导钙流。Lkn-1抑制了由生长因子组合刺激的人类粒细胞-巨噬细胞、红系和多能祖细胞的集落形成。因此，我们分离并鉴定了一种人类C6β-趋化因子，它是CCR1和CCR3的有效激动剂，并具有广泛的生物学活性，包括白细胞趋化作用。
