Reamer R H, Dey B P, White C A, Mageau R P
U.S. Department of Agriculture, Food Safety and Inspection Service, Washington, DC 20250, USA.
J AOAC Int. 1998 Mar-Apr;81(2):398-402.
Standard curves of 5 antibiotics were determined in an antibiotic assay using bilayer and monolayer agar plates and AOAC-specified test organisms and agar media. Micrococcus luteus ATCC 9341a and antibiotic medium No. 2 were used to prepare the penicillin G standard curve. The same organism and antibiotic medium No. 11 were used to prepare the erythromycin standard curve. Standard curves for streptomycin, tetracycline, and gentamicin were prepared, respectively, with antibiotic medium No. 5 and Bacillus subtilis ATCC 6633, antibiotic medium No. 8 and B. cereus ATCC 11778, and antibiotic medium No. 11 and Staphylococcus epidermidis ATCC 12228. Assays of inhibition by meat fortified with penicillin, streptomycin, gentamicin, tetracycline, erythromycin also were performed on monolayer and bilayer plates. Differences in standard curves and inhibitory responses obtained with monolayer and bilayer plates were < 10%. Thus, monolayer plates are acceptable for use in analyses of meat and poultry for antibiotics residues, with savings in laboratory resources and time.
在抗生素检测中，使用双层和单层琼脂平板以及美国官方分析化学师协会（AOAC）指定的测试微生物和琼脂培养基测定了5种抗生素的标准曲线。用藤黄微球菌ATCC 9341a和2号抗生素培养基制备青霉素G标准曲线。用相同的微生物和11号抗生素培养基制备红霉素标准曲线。分别用5号抗生素培养基和枯草芽孢杆菌ATCC 6633、8号抗生素培养基和蜡样芽孢杆菌ATCC 11778、11号抗生素培养基和表皮葡萄球菌ATCC 12228制备链霉素、四环素和庆大霉素的标准曲线。还在单层和双层平板上对添加了青霉素、链霉素、庆大霉素、四环素、红霉素的肉的抑菌作用进行了检测。单层和双层平板获得的标准曲线和抑制反应的差异<10%。因此，单层平板可用于肉类和禽类抗生素残留分析，可节省实验室资源和时间。
