通过液相色谱法测定肉鸡肌肉中的脂质氧化水平。
Determination of lipid oxidation level in broiler meat by liquid chromatography.
作者信息
Marcincák Slavomir, Sokol Jozef, Bystrický Pavel, Popelka Peter, Turek Peter, Bhide Mangesh, Máté Dionýz
机构信息
University of Veterinary Medicine, Department of Food Hygiene and Technology, Komenského 73, 041 81 Kosice, The Slovak Republic.
出版信息
J AOAC Int. 2004 Sep-Oct;87(5):1148-52.
An assay was conducted for the determination of malondialdehyde (MDA) levels in broiler meat. The method involves extraction of tissues with trichloroacetic acid (TCA) and reaction of the TCA extract with 2,4-dinitrophenylhydrazine (DNPH). After separation of the MDA-DNPH complex using a solid-phase extraction C18 column, samples were eluted with 1 mL acetonitrile. Aliquots of 20 microL acetonitrile were analyzed by liquid chromatography on reversed-phase C18 column (3 microm) with UV detection. The products were eluted isocratically with the mobile phase containing acetonitrile-water-acetic acid (39 + 61 + 0.2, v/v/v). The retention time for MDA-DNPH was 6.5 min, and the detection limit was 3.5 microg/kg. Two extraction methods (cold and hot) were also used in the study. The results showed that hot extraction increased results about 55.8% and recovery from samples spiked with 116.6 microg/kg was lower (74.6%) in comparison with cold extraction (94.7%).
进行了一项测定鸡肉中丙二醛（MDA）水平的分析。该方法包括用三氯乙酸（TCA）提取组织，并使TCA提取物与2,4-二硝基苯肼（DNPH）反应。使用固相萃取C18柱分离MDA-DNPH复合物后，用1 mL乙腈洗脱样品。取20 μL乙腈等分试样，在反相C18柱（3 μm）上进行液相色谱分析，并采用紫外检测。产物用含有乙腈-水-乙酸（39 + 61 + 0.2，v/v/v）的流动相等度洗脱。MDA-DNPH的保留时间为6.5分钟，检测限为3.5 μg/kg。该研究还使用了两种提取方法（冷提取和热提取）。结果表明，热提取使结果提高了约55.8%，与冷提取（94.7%）相比，加标量为116.6 μg/kg的样品回收率较低（74.6%）。
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