Mizuki N, Ohno S, Ando H, Sato T, Imanishi T, Gojobori T, Ishihara M, Goto K, Ota M, Geng Z, Geng L, Li G, Inoko H
Department of Ophthalmology, Yokohama City University School of Medicine, Kanagawa, Japan.
Tissue Antigens. 1998 Mar;51(3):287-92. doi: 10.1111/j.1399-0039.1998.tb03104.x.
HLA class II (DRB1, DQA1, DQB1 and DPB1) genotyping was performed in 57 unrelated Uygur individuals inhabiting the northwestern China area by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Among 98 DRB1 alleles tested, 23 alleles were detected, and DRB10701 (16.7%) and DRB10301 (14.0%) were the most and the second most common alleles, respectively. In 8 DQA1 alleles detected, DQA105 (26.3%), DQA103 (21.9%) and DQA10201 (21.1%) were very frequent. Of 21 DQB1 alleles tested, 13 were observed. Among them, DQB102 was highly predominant with the gene frequency of 32.5%. Of 46 DPB1 alleles tested, 15 were detected, among which DPB10401 (31.6%) was the most frequent. Two haplotypes predominate clearly; DRB10701-DQA10201-DQB102 (15.5%) and DRB10301-DQA105-DQB1*02 (12.6%). The dendrogram constructed by the neighbour-joining (NJ) method based on the allele frequencies of the DRB1, DQA1, DQB1 and DPB1 genes of 13 representative populations all over the world suggested that Uygur belonged to the Asian group and lay at the closest genetic distance to a Kazak population inhabiting the same area.
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，对居住在中国西北地区的57名无血缘关系的维吾尔族个体进行了人类白细胞抗原II类（DRB1、DQA1、DQB1和DPB1）基因分型。在检测的98个DRB1等位基因中，共检测到23个等位基因，其中DRB10701（16.7%）和DRB10301（14.0%）分别是最常见和第二常见的等位基因。在检测的8个DQA1等位基因中，DQA105（26.3%）、DQA103（21.9%）和DQA10201（21.1%）出现频率很高。在检测的21个DQB1等位基因中，观察到13个。其中，DQB102占主导地位，基因频率为32.5%。在检测的46个DPB1等位基因中，检测到15个，其中DPB10401（31.6%）出现频率最高。两种单倍型明显占优势；DRB10701-DQA10201-DQB102（15.5%）和DRB10301-DQA105-DQB1*02（12.6%）。基于全世界13个代表性人群的DRB1、DQA1、DQB1和DPB1基因的等位基因频率，采用邻接法（NJ）构建的系统树表明，维吾尔族属于亚洲群体，与居住在同一地区的哈萨克族群体遗传距离最近。
