Ott M, Seidl C, Westhoff U, Stecker K, Seifried E, Fischer P A, Grosse-Wilde H
Picower Institute for Medical Research, Manhasset, New York 11030, USA.
Tissue Antigens. 1998 Mar;51(3):301-4. doi: 10.1111/j.1399-0039.1998.tb03106.x.
Soluble HLA class I (sHLA-I) and soluble HLA class II (sHLA-II) antigen levels during different stages of disease were investigated in paired serum and cerebrospinal fluid (CSF) samples from 37 patients with multiple sclerosis (MS) using ELISA and Western blot analysis. Soluble HLA-II antigens in the serum of untreated patients with the relapsing-remitting type of MS (RRMS) were found to be significantly elevated in acute relapse as compared to values obtained from patients under steroid treatment, in remission or healthy controls. No significant differences in circulating sHLA-I levels could be detected. In contrast, a trend towards increased intrathecal production of sHLA-I molecules in the CSF was observed in untreated RRMS patients in acute relapse, whereas the levels of soluble HLA-II antigens in the CSF were below the detection limit of the ELISA method. Our observations underline the presence of systemic immune activation in MS patients, as reflected in elevated serum sHLA-II antigen levels, and reveal a dichotomy between sHLA class I and II antigen production in the peripheral blood versus CSF in acute MS. Serial measurements of sHLA-II antigen levels might represent a non-invasive method to assess disease activity in MS patients.
利用酶联免疫吸附测定（ELISA）和蛋白质印迹分析，对37例多发性硬化症（MS）患者配对的血清和脑脊液（CSF）样本在疾病不同阶段的可溶性人类白细胞抗原I类（sHLA-I）和可溶性人类白细胞抗原II类（sHLA-II）抗原水平进行了研究。发现复发缓解型多发性硬化症（RRMS）未经治疗患者血清中的可溶性HLA-II抗原在急性复发期较接受类固醇治疗的患者、缓解期患者或健康对照者的值显著升高。未检测到循环sHLA-I水平有显著差异。相反，在急性复发期未经治疗的RRMS患者中，观察到脑脊液中sHLA-I分子鞘内产生增加的趋势，而脑脊液中可溶性HLA-II抗原水平低于ELISA方法的检测限。我们的观察结果强调了MS患者存在全身免疫激活，这反映在血清sHLA-II抗原水平升高，并揭示了急性MS外周血与脑脊液中sHLA I类和II类抗原产生的二分法。连续测量sHLA-II抗原水平可能是评估MS患者疾病活动度的一种非侵入性方法。
