Seymour B W, Pinkerton K E, Friebertshauser K E, Coffman R L, Gershwin L J
Department of Immunobiology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304, USA.
J Immunol. 1997 Dec 15;159(12):6169-75.
Epidemiologic studies have suggested that "second-hand smoke" increases the incidence and severity of allergies and asthma. To address this, we developed a murine model that included generation of and exposure to environmental tobacco smoke (ETS) followed by aerosolized Ag challenge. We report herein that exposure of OVA-sensitized BALB/c mice to ETS elicits a rapid and prolonged exaggerated response with respect to IgE, IgG1, eosinophils, and Th2 cytokines (particularly IL-4 and IL-10). There was a significant increase (p = 0.0159) in the total cells of the bronchoalveolar lavage in OVA-sensitized mice exposed to ETS when compared with those in ambient air. This is the first experimental data to demonstrate that "second-hand smoke" up-regulates the allergic response to inhaled Ag. The results have important health implications for children and allergic individuals residing in households with smokers.
流行病学研究表明，“二手烟”会增加过敏和哮喘的发病率及严重程度。为解决这一问题，我们构建了一种小鼠模型，该模型包括产生环境烟草烟雾（ETS）并使其暴露，随后进行雾化抗原激发。我们在此报告，将卵清蛋白致敏的BALB/c小鼠暴露于ETS会引发针对IgE、IgG1、嗜酸性粒细胞和Th2细胞因子（特别是IL-4和IL-10）的快速且持久的过度反应。与暴露于环境空气的小鼠相比，暴露于ETS的卵清蛋白致敏小鼠支气管肺泡灌洗中的总细胞数显著增加（p = 0.0159）。这是首个证明“二手烟”上调对吸入性抗原过敏反应的实验数据。这些结果对居住在有吸烟者家庭中的儿童和过敏个体具有重要的健康意义。
