Zhang X, Cornelis R, de Kimpe J, Mees L, Lameire N
Laboratory for Analytical Chemistry, Institute for Nuclear Sciences, University of Gent, Belgium.
Clin Chem. 1998 Jan;44(1):141-7.
Arsenic (As) bound to serum proteins in patients on continuous ambulatory peritoneal dialysis (CAPD) was studied. A prior experiment by ultrafiltration showed that 5.57% of total As was bound to serum proteins for 14 CAPD patients. Further identification of the As species and protein molecules in serum of three CAPD patients with high As concentrations was carried out by combining the separation methods of size-exclusion, anion-exchange, and affinity fast-protein liquid chromatography, detected by hydride generation atomic absorption spectrometry. The results indicated that only inorganic As species are bound to serum proteins. Transferrin is the main carrier. The concentrations of As bound to proteins in serum for the three patients were 0.44 +/- 0.12, 0.19 +/- 0.09, and 0.59 +/- 0.09 microg/L (n = 3), respectively.
对持续非卧床腹膜透析（CAPD）患者血清蛋白结合的砷（As）进行了研究。先前的超滤实验表明，14名CAPD患者中，总砷的5.57%与血清蛋白结合。通过结合尺寸排阻、阴离子交换和亲和快速蛋白液相色谱的分离方法，并采用氢化物发生原子吸收光谱法进行检测，对三名砷浓度较高的CAPD患者血清中的砷物种和蛋白质分子进行了进一步鉴定。结果表明，只有无机砷物种与血清蛋白结合。转铁蛋白是主要载体。三名患者血清中与蛋白结合的砷浓度分别为0.44±0.12、0.19±0.09和0.59±0.09μg/L（n = 3）。
