通过离子选择电极和超滤法测量健康个体及持续性非卧床腹膜透析患者血清中的镁组分。

Magnesium fractions in serum of healthy individuals and CAPD patients, measured by an ion-selective electrode and ultrafiltration.

作者信息

Huijgen H J, van Ingen H E, Kok W T, Sanders G T

机构信息

Department of Clinical Chemistry, Academic Medical Centre, Amsterdam, The Netherlands.

出版信息

Clin Biochem. 1996 Jun;29(3):261-6. doi: 10.1016/0009-9120(96)84729-b.

DOI:10.1016/0009-9120(96)84729-b

PMID:8740513

Abstract

OBJECTIVES

Validation and comparison of a magnesium ion-selective electrode (ISE) with a cation-exchange resin technique, followed by determination of all magnesium fractions in serum of healthy volunteers and continuous ambulatory peritoneal dialysis (CAPD) patients.

DESIGN AND METHODS

The analytical aspect has been studied by measuring the influence of complexing agents on the fraction ionized magnesium (friMg2+). A theoretical approximation of friMg2+, based on mass equilibria and complexation constants, was calculated and compared with the measurements.

RESULTS

ISE measurements showed good agreement with theory. Reference values of the ionized, protein-bound, and complexed magnesium fractions were (mean +/- SD) 0.65 +/- 0.04, 0.27 +/- 0.04, and 0.08 +/- 0.03, respectively. Fractions obtained in the CAPD group were 0.62 +/- 0.04, 0.22 +/- 0.05, and 0.16 +/- 0.05, respectively, and differed significantly from the values of the reference population.

CONCLUSIONS

All known serum magnesium parameters can be established by a combination of ultrafiltration, atomic absorption spectrometry, and ISE measurements. Unknown complexing compounds most probably account for the increased fraction of complexed magnesium in the serum of CAPD patients.

摘要