Jessus C, Ozon R
Laboratoire de Physiologie de la Reproduction, INRA/URA-CNRS 1449, Université Pierre et Marie Curie, Paris, France.
Prog Cell Cycle Res. 1995;1:215-28. doi: 10.1007/978-1-4615-1809-9_17.
Activation of the cyclin B-cdc2 kinase mitotic inducer involves dephosphorylation of two inhibitory residues, tyrosine 15 and threonine 14, cdc25 is the specific phosphatase that directly dephosphorylates and activates the cdc2 kinase, cdc25 activity is regulated by phosphorylation. Both phosphatases 1 and 2A could act as cdc25-specific inhibitory phosphatases. Although the cyclin B-cdc2 complex plays a role in activating cdc25, it is highly probable that a distinct protein kinase is involved as a trigger in cdc25 activation. The implication of raf kinase as a cdc25-specific activating kinase in human cells and Xenopus oocytes is discussed.
细胞周期蛋白B - cdc2激酶有丝分裂诱导剂的激活涉及两个抑制性残基（酪氨酸15和苏氨酸14）的去磷酸化，cdc25是直接使cdc2激酶去磷酸化并激活它的特异性磷酸酶，cdc25活性受磷酸化调节。磷酸酶1和2A都可作为cdc25特异性抑制性磷酸酶。虽然细胞周期蛋白B - cdc2复合物在激活cdc25中起作用，但很可能有一种独特的蛋白激酶作为cdc25激活的触发因素。文中讨论了raf激酶作为人细胞和非洲爪蟾卵母细胞中cdc25特异性激活激酶的意义。
