Mendenhall M D, Hodge A E
L. P. Markey Cancer Center, University of Kentucky, Lexington, Kentucky 40536-0096, USA.
Microbiol Mol Biol Rev. 1998 Dec;62(4):1191-243. doi: 10.1128/MMBR.62.4.1191-1243.1998.
The cyclin-dependent protein kinase (CDK) encoded by CDC28 is the master regulator of cell division in the budding yeast Saccharomyces cerevisiae. By mechanisms that, for the most part, remain to be delineated, Cdc28 activity controls the timing of mitotic commitment, bud initiation, DNA replication, spindle formation, and chromosome separation. Environmental stimuli and progress through the cell cycle are monitored through checkpoint mechanisms that influence Cdc28 activity at key cell cycle stages. A vast body of information concerning how Cdc28 activity is timed and coordinated with various mitotic events has accrued. This article reviews that literature. Following an introduction to the properties of CDKs common to many eukaryotic species, the key influences on Cdc28 activity-cyclin-CKI binding and phosphorylation-dephosphorylation events-are examined. The processes controlling the abundance and activity of key Cdc28 regulators, especially transcriptional and proteolytic mechanisms, are then discussed in detail. Finally, the mechanisms by which environmental stimuli influence Cdc28 activity are summarized.
由CDC28编码的细胞周期蛋白依赖性蛋白激酶（CDK）是芽殖酵母酿酒酵母中细胞分裂的主要调节因子。通过在很大程度上仍有待阐明的机制，Cdc28活性控制有丝分裂承诺的时间、芽的起始、DNA复制、纺锤体形成和染色体分离。通过在关键细胞周期阶段影响Cdc28活性的检查点机制来监测环境刺激和细胞周期进程。关于Cdc28活性如何定时以及与各种有丝分裂事件协调的大量信息已经积累起来。本文对该文献进行综述。在介绍了许多真核生物共有的CDK特性之后，研究了对Cdc28活性的关键影响——细胞周期蛋白-CKI结合以及磷酸化-去磷酸化事件。然后详细讨论了控制关键Cdc28调节因子丰度和活性的过程，特别是转录和蛋白水解机制。最后，总结了环境刺激影响Cdc28活性的机制。
