Erb K J, Kirman J, Delahunt B, Chen W, Le Gros G
Malaghan Institute of Medical Research, Wellington, New Zealand.
Immunol Cell Biol. 1998 Feb;76(1):41-6. doi: 10.1046/j.1440-1711.1998.00719.x.
Mycobacterial infections in mice are normally characterized by a profound Th1 cell-mediated immune response, in which T cells secrete large amounts of IFN-gamma. Recent evidence suggests that this response also includes a Th2 component. In order to investigate whether production of IL-4, IL-5, or IL-10 influenced the outcome of a Mycobacterium bovis-bacille Calmette-Guérin (BCG) infection, we intranasally infected IL-4, IL-5, and IL-10 gene-deficient and control mice and monitored the resulting immune response and bacterial clearance. IL-4, IL-5, and IL-10 deficient mice cleared the mycobacteria with the same kinetics as control mice. Furthermore, T cells of cytokine deficient and control mice produced similar levels of IFN-gamma following in vitro stimulation with purified protein derivative (PPD) from M. bovis. We conclude that the cytokines IL-4, IL-5 and IL-10 are not essential for and do not negatively influence the protective immune response against M. bovis-BCG in the lung of mice.
小鼠的分枝杆菌感染通常以深刻的Th1细胞介导的免疫反应为特征，其中T细胞分泌大量的干扰素-γ。最近的证据表明，这种反应还包括一个Th2成分。为了研究白细胞介素-4（IL-4）、白细胞介素-5（IL-5）或白细胞介素-10（IL-10）的产生是否会影响牛分枝杆菌-卡介苗（BCG）感染的结果，我们经鼻感染了IL-4、IL-5和IL-10基因缺陷小鼠和对照小鼠，并监测由此产生的免疫反应和细菌清除情况。IL-4、IL-5和IL-10缺陷小鼠清除分枝杆菌的动力学与对照小鼠相同。此外，细胞因子缺陷小鼠和对照小鼠的T细胞在用来自牛分枝杆菌的纯化蛋白衍生物（PPD）进行体外刺激后，产生的干扰素-γ水平相似。我们得出结论，细胞因子IL-4、IL-5和IL-10对于小鼠肺部针对牛分枝杆菌-卡介苗的保护性免疫反应不是必需的，也不会对其产生负面影响。
