Szczepaniak Z, Gut W, Jarzabek Z
Zakład Wirusologii PZH w Warszawie.
Med Dosw Mikrobiol. 1997;49(3-4):207-14.
The aim of this work was to adapt the Western blot method to analyse the humoral response to proteins of rotavirus strains having various antigens. 10 serum samples from animals immunized with SA11, 18 serum samples from hospitalized children with rotavirus infections and 17 serum samples from healthy adults were examined for rotavirus antibodies using Western blot. Antibodies against all structural rotavirus proteins were detected. The examined sera reacted differently with rotavirus proteins depending on the strain used. The highest IgG reactivity was observed for Wa and SA11, while the lowest was noted for DS-1. At the same time a different level of cross-reactivity of human sera with specific proteins of antigenically varying rotaviruses was observed. The sera taken from adults showed a lower reactivity with proteins of antigens used compared with sera from children. The exception was VP6 of the Ito strain for which the reactivity of adult sera was statistically higher. Essential differences in the reactivity of children's and adult sera were found mainly in the case of VP2 and VP4 belonging to the SA11 strain and VP4 and VP7 of the Ito strain. From these investigations we can conclude that the Western blot method may be useful in assessing immune response caused by rotaviruses. The results of reactivity of some human sera with proteins of strains used indicates that the persons had previously been infected by antigenically varied rotaviruses.
这项工作的目的是调整蛋白质印迹法，以分析对具有不同抗原的轮状病毒株蛋白质的体液反应。使用蛋白质印迹法检测了10份用SA11免疫的动物血清样本、18份住院的轮状病毒感染儿童血清样本和17份健康成年人血清样本中的轮状病毒抗体。检测到了针对所有轮状病毒结构蛋白的抗体。根据所用毒株的不同，检测的血清与轮状病毒蛋白的反应也不同。观察到Wa和SA11的IgG反应性最高，而DS-1的最低。同时，观察到人类血清与抗原性不同的轮状病毒的特定蛋白的交叉反应水平也不同。与儿童血清相比，成人血清与所用抗原蛋白的反应性较低。例外情况是Ito株的VP6，成人血清对其反应性在统计学上更高。儿童和成人血清反应性的本质差异主要存在于属于SA11株的VP2和VP4以及Ito株的VP4和VP7的情况中。从这些研究中我们可以得出结论，蛋白质印迹法可能有助于评估轮状病毒引起的免疫反应。一些人类血清与所用毒株蛋白的反应性结果表明，这些人之前曾感染过抗原性不同的轮状病毒。
