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[在波兰应用蛋白质印迹法进行包虫病血清学诊断]

[Use of western blot methods for serodiagnosis of hydatidosis in Poland].

作者信息

Bitkowska E, Gołab E, Płonka W, Dzbeński T H

机构信息

Zakład Parazytologii Lekarskiej, PZH, Warszawa.

出版信息

Med Dosw Mikrobiol. 1997;49(3-4):215-23.

PMID:9554155
Abstract

46 sera and 1 cerebrospinal fluid samples from 47 patients suspected of hydatidosis were examined by Western blot method, and the results were compared with those of the indirect haemagglutination, immunoelectrophoresis and an enzyme-linked immunosorbent assay (ELISA). The examinations confirmed high sensitivity and specificity of the reactions with 8 kDa antigenic fraction in the Western blot. The above data supported the concept, that serological diagnosis of hydatidosis should be carried out using a high sensitivity screening test, such as ELISA, then the Western blot method to verify positive results.

摘要

采用蛋白质印迹法对47例疑似包虫病患者的46份血清和1份脑脊液样本进行检测,并将结果与间接血凝试验、免疫电泳和酶联免疫吸附测定(ELISA)的结果进行比较。检测证实了蛋白质印迹法中与8 kDa抗原组分反应具有高敏感性和特异性。上述数据支持这样一种观念,即包虫病的血清学诊断应先用ELISA等高敏感性筛查试验,然后用蛋白质印迹法验证阳性结果。

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