Van Regenmortel M H, Guichard G, Benkirane N, Briand J P, Muller S, Brown F
Institut de Biologie Moléculaire et Cellulaire, CNRS UPR, Strasbourg, France.
Dev Biol Stand. 1998;92:139-43.
Retro-inverso (RI) peptides, also called all-D-retro peptides, have been shown to mimic the antigenic and immunogenic properties of L-peptides successfully. RI peptides corresponding to the loop 141-159 of the VP1 protein of foot-and-mouth disease virus (FMDV) have been synthesized and used to immunize rabbits and guinea pigs. These peptides induced longer-lasting and higher antibody titres in immunized animals than did the corresponding L-peptides and the antibodies cross-reacted strongly with virus particles and with L-peptides. Antisera raised to RI peptides had in vitro virus neutralization titres equal to or better than those obtained after immunization with classical FMDV antigens and L-peptides. In view of their increased stability, RI peptides may overcome some of the shortcomings of synthetic viral vaccines based on L-peptides.
反向内消旋（RI）肽，也称为全D-反向肽，已被证明能够成功模拟L-肽的抗原性和免疫原性。已合成了与口蹄疫病毒（FMDV）VP1蛋白141-159环对应的RI肽，并用于免疫兔子和豚鼠。与相应的L-肽相比，这些肽在免疫动物中诱导出更持久、更高的抗体滴度，并且抗体与病毒颗粒和L-肽强烈交叉反应。用RI肽产生的抗血清的体外病毒中和滴度等于或优于用经典FMDV抗原和L-肽免疫后获得的滴度。鉴于其稳定性增强，RI肽可能克服基于L-肽的合成病毒疫苗的一些缺点。
