Muller S, Guichard G, Benkirane N, Brown F, Van Regenmortel M H, Briand J P
Institut de Biologie Moléculaire et Cellulaire, UPR 9021 CNRS, Strasbourg, France.
Pept Res. 1995 May-Jun;8(3):138-44.
Retro-inverso analogues of peptides corresponding to the major antigenic site 141-159 of VP1 from two foot-and-mouth disease virus variants have been synthesized and tested for their antigenic and immunogenic properties. Antibodies to the L- and retro-inverso peptides were produced by injecting rabbits with peptides covalently coupled to small unilamellar liposomes containing monophosphoryl lipid A as adjuvant. When compared to the antibody response raised against the L-peptides, the duration of the IgG response that was induced by the retro-inverso peptides was significantly longer and the titer of anti-peptide antisera was much higher. Antibodies to retro-inverso peptides cross-reacted equally well with the respective parent L-peptides. These results, obtained with a viral sequence which was found previously to represent a good candidate for possible vaccination, show that retro-inverso peptidomimetics could be useful for enhancing the immunogenicity of peptides.
已合成了与两种口蹄疫病毒变体的VP1主要抗原位点141 - 159相对应的肽的反向模拟类似物，并对其抗原性和免疫原性进行了测试。通过向兔子注射与含有单磷酰脂质A作为佐剂的小单层脂质体共价偶联的肽，产生了针对L - 肽和反向模拟肽的抗体。与针对L - 肽引发的抗体反应相比，反向模拟肽诱导的IgG反应持续时间显著更长，抗肽抗血清的效价更高。针对反向模拟肽的抗体与各自的亲本L - 肽交叉反应同样良好。这些结果是用先前发现可能是潜在疫苗良好候选物的病毒序列获得的，表明反向模拟肽模拟物可用于增强肽的免疫原性。
