Inchauspé G, Major M E, Nakano I, Vivitski L, Maisonnas M, Trépo C
INSERM, U271, Lyon, France.
Dev Biol Stand. 1998;92:163-8.
We have used direct DNA inoculation to study the in vivo induction of both humoral and cellular immune responses to hepatitis C virus (HCV) encoded structural antigens. Following immunisation of mice, immune responses were compared using plasmids encoding full-length or partial HCV gene sequences for the nucleocapsid and envelope E2 proteins. Plasmids encoding secreted or non-secreted forms of the immunogens, including constructs expressing HCV sequences fused with the hepatitis B virus surface antigen (HCV-HBV chimeras), were evaluated. Results indicate that: (i) all constructs induced specific anti-HCV antibodies; (ii) antibody titres ranged from 1:100 to > 1:100,000; (iii) all HCV DNA immunogens induced a predominant Th1 response with the induction of IgG2a antibodies; (iv) the secretion level of the antigens and immune responses was not always correlated and (v) CTL could be detected against both HCV and HBV determinants.
我们已采用直接DNA接种法来研究针对丙型肝炎病毒（HCV）编码的结构抗原的体液免疫和细胞免疫反应在体内的诱导情况。给小鼠免疫后，使用编码核衣壳和包膜E2蛋白的全长或部分HCV基因序列的质粒比较免疫反应。对编码免疫原的分泌型或非分泌型形式的质粒进行了评估，包括表达与乙型肝炎病毒表面抗原融合的HCV序列的构建体（HCV-HBV嵌合体）。结果表明：（i）所有构建体均诱导出特异性抗HCV抗体；（ii）抗体效价范围为1:100至>1:100,000；（iii）所有HCV DNA免疫原均诱导出以IgG2a抗体诱导为主的Th1反应；（iv）抗原的分泌水平与免疫反应并不总是相关；（v）可检测到针对HCV和HBV决定簇的细胞毒性T淋巴细胞（CTL）。
