Wan L, Hubbard R W
Department of Pathology & Laboratory Medicine, School of Medicine, Loma Linda University, CA 92350, USA.
Clin Chem. 1998 Apr;44(4):810-6.
Carnitine deficiency presents as a major problem in fatty acid oxidation. The use of a plasma carnitine assay can rapidly help to describe this deficiency. The method we describe here requires two simple steps of sample preparation, followed by automated analysis with the Beckman Synchron CX4 random-access chemistry analyzer. The goal of this method development was to reduce the cost of analysis and to allow a greater number of laboratories to perform this assay on demand within 1 h for both free and total carnitine. The method has a linearity of 0-150 micromol/L and a detection limit of 5 micromol/L. The inter- and intraday CVs are <20%. The method agreed closely with both the widely used RIA and spectrophotometric methods.
肉碱缺乏是脂肪酸氧化中的一个主要问题。血浆肉碱检测有助于快速诊断这种缺乏症。我们在此描述的方法需要两个简单的样品制备步骤，然后使用贝克曼同步CX4随机存取化学分析仪进行自动分析。该方法开发的目的是降低分析成本，并使更多实验室能够在1小时内按需进行游离和总肉碱检测。该方法的线性范围为0-150微摩尔/升，检测限为5微摩尔/升。日间和日内变异系数均<20%。该方法与广泛使用的放射免疫分析法和分光光度法结果高度一致。
