Seccombe D W, Dodek P, Frohlich J, Hahn P, Skala J P, Campbell D J
Clin Chem. 1976 Oct;22(10):1589-92.
Because of renewed interest in a possible connection between carnitine, lipid disorders, and myopathy, an automated method of analysis is desirable. Deproteinization of serum by use of membrane filter cones and automated assay with a bichromatic analyzer (the ABA-100) substantially increases efficiency without sacrificing the specificity and accuracy of the original manual enzymatic method. The described procedure allows for analysis of 80 speciments a day and is thus suitable for screening of selected populations. Normal values found in blood sera of adults were in the range of 25.0-73.8 mu mol/liter and the method has sufficient sensitivity to accurately measure concentrations as small as 10 mu mol/liter.
由于对肉碱、脂质紊乱和肌病之间可能存在的联系重新产生了兴趣，因此需要一种自动化分析方法。使用膜滤锥对血清进行脱蛋白处理，并使用双色分析仪（ABA - 100）进行自动化检测，在不牺牲原始手动酶法特异性和准确性的前提下，大幅提高了效率。所描述的方法每天可分析80个样本，因此适用于对特定人群的筛查。在成人血清中发现的正常值范围为25.0 - 73.8微摩尔/升，该方法具有足够的灵敏度，能够准确测量低至10微摩尔/升的浓度。
