Numata C, Yokota M, Hosono M, Ogawa Y, Suzuki T, Takayanagi Y, Nitta K
Cancer Research Institute, Tohoku College of Pharmaceutical Sciences, Sendai, Japan.
Biol Pharm Bull. 1998 Mar;21(3):214-8. doi: 10.1248/bpb.21.214.
Physarumin, a carbohydrate-binding protein (hemagglutinin or lectin), was isolated from the plasmodium of Physarum polycephalum. Physarumin agglutinated not only several species of erythrocytes but also tumor cells such as AH109A ascites hepatoma cells, sarcoma 180 ascites cells and mouse leukemia P388 cell lines. Physarumin had tumor cell growth-inhibitory activity, and induced the apoptosis of P388 cell lines. Physarumin-induced apoptosis required binding to a 68 kDa counter-receptor on the P388 cell surface. Since the agglutinating and antiproliferative activities of physarumin were inhibited by asialofetuin and thyroglobulin, respectively, it is suggested that physarumin reacts with the galactose moiety of carbohydrate chains of physarumin receptor.
绒泡菌凝集素是一种碳水化合物结合蛋白（血凝素或凝集素），从多头绒泡菌的原质团中分离得到。绒泡菌凝集素不仅能凝集多种红细胞，还能凝集肿瘤细胞，如AH109A腹水肝癌细胞、肉瘤180腹水细胞和小鼠白血病P388细胞系。绒泡菌凝集素有肿瘤细胞生长抑制活性，并能诱导P388细胞系凋亡。绒泡菌诱导的凋亡需要与P388细胞表面的一种68 kDa反受体结合。由于绒泡菌凝集素的凝集活性和抗增殖活性分别被去唾液酸胎球蛋白和甲状腺球蛋白抑制，提示绒泡菌凝集素与绒泡菌凝集素受体碳水化合物链的半乳糖部分发生反应。
