Lemonnier F, Gautier M, Moatti N, Lemonnier A
In Vitro. 1976 Jun;12(6):460-6. doi: 10.1007/BF02806026.
Amino acid concentration are studied in the extracellular media of ten series of human fibroblast and liver cell monolayer cultures. These two cell types consume and produce ostensively the same amino acids. Among the nonessential amino acids, the most significant variations involve serine and aspartate which are decreased; alpha-alanine, glutamate, ornithine and proline are, on the contrary, increased. Among the essential amino acids, leucine, isoleucine and glutamine are preferentially decreased. The variations of some amino acids are correlated with the cell density. The interrelations which may exist between the variations of these different amino acids are discussed. Furthermore, the glycolytic acitivity of the cells studied is very high: 85% of glucose consumed is found in the form of lactate.
在十组人成纤维细胞和肝细胞单层培养物的细胞外培养基中研究了氨基酸浓度。这两种细胞类型消耗和产生的氨基酸种类大致相同。在非必需氨基酸中，最显著的变化涉及丝氨酸和天冬氨酸，它们的含量降低；相反，α-丙氨酸、谷氨酸、鸟氨酸和脯氨酸含量增加。在必需氨基酸中，亮氨酸、异亮氨酸和谷氨酰胺优先减少。一些氨基酸的变化与细胞密度相关。讨论了这些不同氨基酸变化之间可能存在的相互关系。此外，所研究细胞的糖酵解活性非常高：消耗的葡萄糖中有85%以乳酸的形式存在。
