Abate O, Bollo E, Lotti D, Bo S
University of Turin, Department of Animal Pathology, Italy.
Zentralbl Veterinarmed B. 1998 Mar;45(2):73-85. doi: 10.1111/j.1439-0450.1998.tb00769.x.
Cerebrospinal fluid (CSF) obtained from 20 clinically healthy dogs and from 15 dogs affected by neurological disorders were examined for total and differential cell counts, immunocytochemistry for canine distemper virus antigen, total protein concentration and electrophoretic separation, and glucose and enzyme determination. Dogs affected by canine distemper showed an increase in macrophages, presence of specific inclusion bodies, and an increase in total protein concentration and gamma-globulin fraction. In bacterial meningoencephalomyelitis pleocytosis, increase of total proteins with special regard to high molecular weight proteins, elevated levels of lactate dehydrogenase (LDH), alterations of LDH isoenzyme profile, and decrease in glucose levels were detected; inflammatory disorders were more often characterized by an increase in LDH level, while in non-inflammatory disorders (hydrocephalus and spinal cord neoplasia) no variation in LDH levels was detected. Analysis of CSF in dogs appears relatively easy to perform and may help in establishing the condition of the blood-brain barrier as well as in the diagnosis of neurological disorders.
对从20只临床健康犬和15只患有神经系统疾病的犬获取的脑脊液（CSF）进行了细胞总数和分类计数、犬瘟热病毒抗原免疫细胞化学检测、总蛋白浓度和电泳分离，以及葡萄糖和酶的测定。患有犬瘟热的犬巨噬细胞增多、出现特异性包涵体、总蛋白浓度和γ-球蛋白组分增加。在细菌性脑膜脑脊髓炎的细胞增多症中，检测到总蛋白增加，尤其是高分子量蛋白增加、乳酸脱氢酶（LDH）水平升高、LDH同工酶谱改变以及葡萄糖水平降低；炎症性疾病更常表现为LDH水平升高，而在非炎症性疾病（脑积水和脊髓肿瘤）中未检测到LDH水平变化。对犬脑脊液的分析似乎相对容易进行，并且可能有助于确定血脑屏障的状况以及神经系统疾病的诊断。
