el-Mansy A, Molnár K
Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest, Hungary.
Acta Vet Hung. 1997;45(4):427-38.
The extrapiscine development of Myxobolus drjagini, a myxosporean parasite of the head, operculum and buccal cavity of the silver carp (Hypophthalmichthys molitrix), was studied in experimentally infected oligochaetes, Tubifex tubifex. After infection of uninfected tubificids with mature spores of M. drajagini, development of actinosporean stages was first observed by light microscopy 27 days after infection. Triactinomyxon stages of M. drjagini emerged from the worms after 91 days of intraoligochaete development. In histological sections, early pansporocysts were found in the gut epithelium of the experimental oligochaetes 42 days after infection. Mature pansporocysts, each containing 8 triactinomyxons, appeared 79 days after infection. After rupture of the epithelial cell and the pansporocyst, free actinosporean stages were found in the gut lumen of the oligochaete. Actinosporean stages released from oligochaetes appeared in the water 91 days after infection. They were floating in the water and showed a typical triactinomyxon form. Each triactinomyxon had three pyriform polar capsules, a sporoplasm with 14 secondary cells inside the spore body, a moderately long style and slightly bent, trifurcated, conically ending tails. The total length of the triactinomyxon measured approximately 198 microns. The prevalence of infection in 51 oligochaetes proved to be 9.8%. No infection was found in the control oligochaetes.
对鲢（Hypophthalmichthys molitrix）头部、鳃盖和口腔的粘孢子虫寄生虫——德拉吉尼碘泡虫（Myxobolus drjagini）在鱼体外的发育过程进行了研究，实验对象为受感染的寡毛纲颤蚓（Tubifex tubifex）。用德拉吉尼碘泡虫的成熟孢子感染未感染的颤蚓后，感染27天后通过光学显微镜首次观察到放射孢子虫阶段的发育。在寡毛纲内发育91天后，德拉吉尼碘泡虫的三极虫阶段从虫体中出现。在组织学切片中，感染42天后在实验颤蚓的肠道上皮中发现早期全孢子囊。感染79天后出现成熟的全孢子囊，每个全孢子囊含有8个三极虫。上皮细胞和全孢子囊破裂后，在颤蚓的肠腔中发现游离的放射孢子虫阶段。感染91天后，从颤蚓释放的放射孢子虫阶段出现在水中。它们漂浮在水中，呈典型的三极虫形态。每个三极虫有三个梨形极囊，孢子体内有一个含有14个次生细胞的孢质，一个中等长度的极丝和略微弯曲、三叉、锥形末端的尾。三极虫的总长度约为198微米。51条颤蚓的感染率为9.8%。在对照颤蚓中未发现感染。
