Ruidisch S, el-Matbouli M, Hoffmann R W
Institute of Zoology and Hydrobiology, University of Munich, Federal Republic of Germany.
Parasitol Res. 1991;77(8):663-7. doi: 10.1007/BF00928680.
Myxobolus pavlovskii from the gills of silver carp (Hypophthalmichthys molitrix) were used in attempts to transmit the infection under laboratory conditions. Spores of M. pavlovskii were placed in aquaria that contained sterilized sand and had been filled with tap water at 15-16 degrees C. Furthermore, oligochaetes (90% Tubifex tubifex) were added and examined by wet mounts and histology. Hexactinomyxon spores developed after about 3 months only in T. tubifex that had been exposed to M. pavlovskii. Myxosporea-free silver carp developed cysts in their gills that contained M. pavlovskii at 120 days after contact with Hexactinomyxon spores, proving that M. pavlovskii also needs an oligochaete intermediate host for its development.
取自鲢鱼(Hypophthalmichthys molitrix)鳃部的帕氏粘体虫(Myxobolus pavlovskii)被用于尝试在实验室条件下传播感染。将帕氏粘体虫的孢子置于装有灭菌沙子且已注入15 - 16摄氏度自来水的水族箱中。此外,添加了寡毛纲动物(90%为颤蚓(Tubifex tubifex)),并通过湿片法和组织学进行检查。大约3个月后,六放孢子虫的孢子仅在接触过帕氏粘体虫的颤蚓中发育。无粘孢子虫的鲢鱼在与六放孢子虫孢子接触120天后,其鳃部出现含有帕氏粘体虫的囊肿,证明帕氏粘体虫的发育也需要寡毛纲动物作为中间宿主。