Pollack S B, Nelson K
Int J Cancer. 1976 Aug 15;18(2):250-4. doi: 10.1002/ijc.2910180216.
Antisera taken 1 or 2 days after inoculation of BALB/c mice with transplantable sarcoma cells or Moloney sarcoma virus (MSV) induce tumor-specific cell-dependent cytotoxicity in vitro. In the present experiments, lethally irradiated ("immunosuppressed") mice were tested for the early appearance of the serum factor responsible for this anti-serum-dependent cell-mediated cytotoxicity (E-ADC). BALB/c mice were infected with MSV, syngeneic sarcoma cells or sheep red blood cells 24 h following irradiation with 900 R. Sera were obtained from MSV and tumor cell recipients 48 or 72 h later and tested for E-ADC activity. Spleen cells from SRBC recipients were tested at day 4 or 5 for ability to form direct plaques in a modified Jerne plague assay. Although the anti-SRBC response was obliterated in the irradiated mice, the E-ADC response appeared to be unimpaired. These studies indicate that newly synthesized immunoglobulin is not required for the formation of the E-ADC factor.
在用可移植肉瘤细胞或莫洛尼肉瘤病毒（MSV）接种BALB/c小鼠1或2天后采集的抗血清，可在体外诱导肿瘤特异性细胞依赖性细胞毒性。在本实验中，对经致死性照射（“免疫抑制”）的小鼠进行检测，以观察负责这种抗血清依赖性细胞介导细胞毒性（E-ADC）的血清因子的早期出现情况。用900拉德的辐射照射BALB/c小鼠24小时后，使其感染MSV、同基因肉瘤细胞或绵羊红细胞。48或72小时后，从感染MSV和肿瘤细胞的小鼠体内获取血清，并检测其E-ADC活性。在第4或5天，检测来自感染绵羊红细胞小鼠的脾细胞在改良的杰尔恩氏空斑试验中形成直接空斑的能力。尽管照射后的小鼠抗绵羊红细胞反应被消除，但E-ADC反应似乎未受损害。这些研究表明，E-ADC因子的形成不需要新合成的免疫球蛋白。
