Nelson K, Pollack S B, Hellström K E
Int J Cancer. 1975 Aug 15;16(2):292-300. doi: 10.1002/ijc.2910160212.
Sera from tumor-bearing mice induce specific cytotoxicity to tumor cells by non-immune lymphoid cells (antiserum-dependent cytotoxicity or ADC). When spleen cells from BALB/c mice bearing autochthonous or syngeneic sarcomas were cultured in vitro, culture supernatants were obtained which specifically sensitized sarcoma cells to injury in vitro by normal lymph-node cells (LNC). Culture supernatants of spleen cells from mice whose transplanted sarcomas had been excised also induced ADC. The ADC activity resided in the mouse immunoglobulin fraction of the culture supernatants and its synthesis did not depend on the presence of theta-positive cells. Following a brief in vivo exposure to culture supernatant with known ADC activity, LNC from non-immune mice specifically destroyed tumor cells in an in vitro assay.
荷瘤小鼠的血清可通过非免疫淋巴细胞诱导对肿瘤细胞的特异性细胞毒性（抗血清依赖性细胞毒性或 ADC）。当将携带自体或同基因肉瘤的 BALB/c 小鼠的脾细胞进行体外培养时，可获得培养上清液，该上清液能使肉瘤细胞在体外对正常淋巴结细胞（LNC）的损伤产生特异性敏感。已切除移植肉瘤的小鼠脾细胞培养上清液也可诱导 ADC。ADC 活性存在于培养上清液的小鼠免疫球蛋白组分中，其合成不依赖于 θ 阳性细胞的存在。在体内短暂接触具有已知 ADC 活性的培养上清液后，来自非免疫小鼠的 LNC 在体外试验中可特异性破坏肿瘤细胞。
