Akiyoshi D E, Denaro M, Zhu H, Greenstein J L, Banerjee P, Fishman J A
BioTransplant, Incorporated, Charlestown, Massachusetts, USA.
J Virol. 1998 May;72(5):4503-7. doi: 10.1128/JVI.72.5.4503-4507.1998.
Endogenous retroviruses of swine are a concern in the use of pig-derived tissues for xenotransplantation into humans. The nucleotide sequence of porcine endogenous retrovirus taken from lymphocytes of miniature swine (PERV-MSL) has been characterized. PERV-MSL is a type C retrovirus of 8,132 bp with the greatest nucleic acid sequence identity to gibbon ape leukemia virus and murine leukemia virus. Constitutive production of PERV-MSL RNA has been detected in normal leukocytes and in multiple organs of swine. The copy numbers of full-length PERV sequences per genome (approximately 8 to 15) vary among swine strains. The open reading frames for gag, pol, and env in PERV-MSL have over 99% amino acid sequence identity to those of Tsukuba-1 retrovirus and are highly homologous to those of endogenous retrovirus of cell line PK15 (PK15-ERV). Most of the differences in the predicted amino acid sequences of PK15-ERV and PERV-MSL are in the SU (cell attachment) region of env. The existence of these PERV clones will enable studies of infection by endogenous retroviruses in xenotransplantation.
猪内源性逆转录病毒是猪源组织用于异种移植到人体时需要关注的问题。已对从小型猪淋巴细胞中提取的猪内源性逆转录病毒(PERV-MSL)的核苷酸序列进行了表征。PERV-MSL是一种8132 bp的C型逆转录病毒,与长臂猿白血病病毒和鼠白血病病毒的核酸序列同一性最高。在正常白细胞和猪的多个器官中已检测到PERV-MSL RNA的组成型产生。每个基因组中全长PERV序列的拷贝数(约8至15个)在猪品系间有所不同。PERV-MSL中gag、pol和env的开放阅读框与筑波-1逆转录病毒的氨基酸序列同一性超过99%,并且与细胞系PK15的内源性逆转录病毒(PK15-ERV)高度同源。PK15-ERV和PERV-MSL预测氨基酸序列的大多数差异存在于env的SU(细胞附着)区域。这些PERV克隆的存在将有助于研究异种移植中内源性逆转录病毒的感染情况。