Stein A, Kula M R, Elling L
Institute of Enzyme Technology, Heinrich-Heine-University Düsseldorf in the research centre Jülich, Germany.
Glycoconj J. 1998 Feb;15(2):139-45. doi: 10.1023/a:1006912121278.
dTDP-6-deoxy-4-keto-D-glucose (1), the common intermediate in the biosyntheses of the manifold deoxysugars, was synthesized on a gram-scale by the combination of sucrose synthase and dTDP-D-glucose 4,6-dehydratase in a fed batch, starting the reaction with dTDP. This process allowed a dTDP conversion with a 100% rate. An easy and efficient three-step purification with anion-exchange chromatography and gel filtration gave 1.1 g of 1 in an overall yield of 73%. This work realizes a first step for an economic access to activated deoxysugars.
dTDP-6-脱氧-4-酮-D-葡萄糖（1）是多种脱氧糖生物合成中的常见中间体，通过在补料分批培养中结合蔗糖合酶和dTDP-D-葡萄糖4,6-脱水酶，以dTDP起始反应，克级规模合成了该中间体。此过程实现了100%的dTDP转化率。通过阴离子交换色谱和凝胶过滤进行的简单高效三步纯化，得到1.1克1，总产率为73%。这项工作实现了经济获取活化脱氧糖的第一步。
