Torii M, Matsumoto Y, Kamboj K K, Maracic M, Guo S Q, Nussenzweig R S, Aikawa M, Cochrane A H
Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106.
Infect Immun. 1989 Feb;57(2):596-601. doi: 10.1128/iai.57.2.596-601.1989.
The localization of Plasmodium brasilianum antigens, common to merozoite micronemes and parasite-induced structures in the host erythrocyte, was determined by means of immunogold electron microscopy and monoclonal antibodies directed against blood stages of this parasite. All monoclonal antibodies reacted with micronemes. In addition, some reacted with either knob protrusions or caveolae of the host erythrocyte membrane; one reacted with a parasite-derived antigen present in the erythrocyte cytoplasm. Gold particles appeared over the membranes of ring-infected cells before the appearance of knobs and caveolae. We hypothesize that at least some knob- and caveolae-associated antigens of P. brasilianum are inserted into the erythrocyte membrane at the time of merozoite invasion.
通过免疫金电子显微镜和针对该寄生虫血液阶段的单克隆抗体，确定了巴西疟原虫抗原在裂殖子微线体和宿主红细胞中寄生虫诱导结构中的定位。所有单克隆抗体均与微线体发生反应。此外，一些抗体与宿主红细胞膜的凸起或小窝发生反应；一种抗体与存在于红细胞胞质中的寄生虫衍生抗原发生反应。在凸起和小窝出现之前，金颗粒出现在环状感染细胞的膜上。我们推测，巴西疟原虫至少一些与凸起和小窝相关的抗原在裂殖子入侵时插入红细胞膜。
