干燥综合征患者外周血单个核细胞上Fcγ受体III（CD16）表达降低。
Decreased Fcgamma receptor III (CD16) expression on peripheral blood mononuclear cells in patients with Sjögren's syndrome.
作者信息
Saito K, Abe T, Takeuchi T
机构信息
2nd Department of Internal Medicine, Saitama Medical Center, Saitama Medical School, Japan.
出版信息
J Rheumatol. 1998 Apr;25(4):689-96.
OBJECTIVE
To examine the expression of surface structures important in natural killer (NK) cell function and the roles of serum factors affecting the expression of surface antigens on these cells in patients with Sjögren's syndrome (SS).
METHODS
Peripheral blood mononuclear cells (PBMC) of 18 patients with SS were analyzed by immunofluorescence on a flow cytometer. The antigen recognized by autoantibodies in their sera was analyzed by immunofluorescence and by immunoprecipitation.
RESULTS
Expression of Fcgamma receptor III (CD16), an important indicator of NK cytolytic activity, on PBMC was significantly decreased in patients with SS who have extraglandular disease, while the expression of Fcgamma RI and II was normal. Moreover, F(ab')2 of IgG in these patients' sera was shown to bind to CD16+ cells. Immunoprecipitation study showed that it bound to CD16 itself.
CONCLUSION
The depressed expression of CD16 on PBMC and the presence of antibody binding to CD16 in the patients' sera may be, at least in part, responsible for the altered function of NK cells observed in SS.
目的
研究干燥综合征（SS）患者中对自然杀伤（NK）细胞功能至关重要的表面结构的表达情况，以及影响这些细胞表面抗原表达的血清因子的作用。
方法
采用流式细胞仪免疫荧光法分析18例SS患者外周血单个核细胞（PBMC）。通过免疫荧光和免疫沉淀法分析其血清中自身抗体识别的抗原。
结果
有腺外病变的SS患者PBMC上NK细胞溶解活性的重要指标Fcγ受体III（CD16）的表达显著降低，而Fcγ RI和II的表达正常。此外，这些患者血清中IgG的F(ab')2与CD16+细胞结合。免疫沉淀研究表明它与CD16本身结合。
结论
PBMC上CD16表达降低以及患者血清中存在与CD16结合的抗体可能至少部分导致了SS患者中观察到的NK细胞功能改变。
Zotero 插件