单价阳离子和精胺刺激大鼠肝脏中缬氨酰转移核糖核酸的形成。
Valyl-transfer RNA formation stimulated by monovalent cations and spermine in rat liver.
作者信息
Romano M, Lombardi M L
出版信息
Ital J Biochem. 1976 May-Jun;25(3):245-50.
PMID:955857
Abstract
Valyl-tRNA synthetase from rat liver catalyzes the aminoacylation of tRNA in the presence of a) Mg2+, b) monovalent cations and c) spermine. The degree of aminoacylation with Na+ is equal to that with Mg2+ but the ATP required with Na+ is 25 times lower than that with Mg2+. The aminoacylation becomes almost undetectable in the presence of Na+ when tRNA deprived of Mg2+ is used.
摘要
来自大鼠肝脏的缬氨酰 - tRNA合成酶在以下条件下催化tRNA的氨酰化反应：a) Mg2+、b) 单价阳离子和c) 精胺。Na+存在时的氨酰化程度与Mg2+存在时相同，但Na+所需的ATP比Mg2+低25倍。当使用去除Mg2+的tRNA时，在Na+存在下氨酰化几乎无法检测到。
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