Tecu C, Gherghina I, Constantinescu C, Chiriţă C, Ioniţă E, Alexandrescu V, Maţepiuc M, Lupulescu E, Vizitiu O
Cantacuzino Institute, Bucharest, Romania.
Roum Arch Microbiol Immunol. 1996 Oct-Dec;55(4):333-9.
114 children with acute respiratory infections were investigated to detect the viral etiological agents. The following methods were used: the direct immunofluorescence technique for the rapid diagnosis from tracheobronchial aspirates and the serological reactions--hemaglutinoinhibition and complement fixation reaction--to determine the increase of antibodies titres. Of 55.2% respiratory infections with a viral etiology, 60.3% represent acute infections of the lower respiratory tract. Due to the influenza epidemic reported between October 1996 and February 1997 (with a higher number of cases than in the last 5 years), most viral respiratory infections were caused by influenza viruses (57.14%). The direct immunofluorescence technique proved to be sensitive and specific in detecting the viral etiological agents which caused respiratory infections. Thus, using this technique, a positive diagnosis was made in 68.2% of cases, of which 49.2% were positive by the hemagglutination and complement fixation reactions, too. The test specificity could have been higher if the pathological products had been prelevated within the first three days after the onset of the disease.
对114名急性呼吸道感染儿童进行了调查，以检测病毒病原体。采用了以下方法：通过直接免疫荧光技术对气管支气管吸出物进行快速诊断，并通过血清学反应——血凝抑制和补体结合反应——来确定抗体滴度的升高。在55.2%的病毒病因性呼吸道感染中，60.3%为下呼吸道急性感染。由于1996年10月至1997年2月期间报告了流感流行（病例数高于过去5年），大多数病毒性呼吸道感染由流感病毒引起（57.14%）。直接免疫荧光技术在检测引起呼吸道感染的病毒病原体方面被证明是敏感和特异的。因此，使用该技术，68.2%的病例做出了阳性诊断，其中49.2%的病例血凝和补体结合反应也呈阳性。如果在疾病发作后的头三天内采集病理产物，检测特异性可能会更高。
