Sainte-Laudy J
Unité d'immuno-allergologie, Lavoratoire Cerba, Cergy-Pontoise.
Allerg Immunol (Paris). 1998 Feb;30(2):41-3.
Among the different methods used for the analysis of human basophil activation as histamine and leukotriene release, flow cytometry presents several methodological advantages. Moreover, preliminary results obtained for a wide allergen panel have demonstrated its high sensitivity and specificity. We present here the results of the methods used to validate the flow cytometric analysis of human basophil activation by double anti-IgE FITC/anti-CD63 PE labelling of human leucocytes. The use of multiple labelling by monoclonals having different specificities have shown that the selected cells express the phenotype CD14-, CD19-, CD41- or +/-, CD45+, CD63- or +. This phenotype is related to human basophils. We have also demonstrated that the expression of CD63 is related to basophil activation and not to aggregated platelets which are also able to express this marker.
在用于分析人类嗜碱性粒细胞活化（如组胺和白三烯释放）的不同方法中，流式细胞术具有若干方法学优势。此外，针对多种过敏原检测获得的初步结果已证明其具有高灵敏度和特异性。我们在此展示了通过人白细胞的双抗IgE FITC/抗CD63 PE标记来验证人类嗜碱性粒细胞活化的流式细胞术分析方法的结果。使用具有不同特异性的单克隆抗体进行多重标记表明，所选细胞表达CD14-、CD19-、CD41- 或 +/ -、CD45 +、CD63 - 或 + 的表型。这种表型与人类嗜碱性粒细胞相关。我们还证明，CD63的表达与嗜碱性粒细胞活化有关，而与同样能够表达该标志物的聚集血小板无关。
