Sainte-Laudy J, Vallon C, Guérin J C
Département des maladies respiratoires, Hôpital de la Croix-Rousse, Lyon.
Allerg Immunol (Paris). 1994 Jun;26(6):211-4.
On the basis of the CD 63 bi-modal expression on the membrane of activated basophils, we set up a flow cytometric method for the analysis of human basophils activation by an anti-IgE and anti-CD 63 double labelling. We demonstrated that the statistical characteristics of the percentages of activation obtained by an anti-IgE stimulation allowed the use of this method for pharmacological studies. The percentages of activation were of the same order of magnitude than those obtained by histamine release. CD 63 expression was also observed for a low affinity allergen such as the sulfonyl-HSA conjugate used for sulfites hypersensibility diagnosis, healthy donors being negative. This method, which can be automatized may represent an interesting candidate in the field of hapten hypersensitivity which lacks of reliable diagnostical methods.
基于活化嗜碱性粒细胞膜上CD 63的双峰表达，我们建立了一种通过抗IgE和抗CD 63双重标记来分析人嗜碱性粒细胞活化的流式细胞术方法。我们证明，通过抗IgE刺激获得的活化百分比的统计特征使得该方法可用于药理学研究。活化百分比与通过组胺释放获得的百分比处于相同的数量级。对于低亲和力变应原，如用于亚硫酸盐超敏反应诊断的磺酰-HSA缀合物，也观察到了CD 63表达，而健康供体为阴性。这种可自动化的方法可能是半抗原超敏反应领域中一个有趣的候选方法，因为该领域缺乏可靠的诊断方法。
