Planta R J, Mager W H
Department of Biochemistry and Molecular Biology, BioCentrum Amsterdam, Vrije Universiteit, The Netherlands.
Yeast. 1998 Mar 30;14(5):471-7. doi: 10.1002/(SICI)1097-0061(19980330)14:5<471::AID-YEA241>3.0.CO;2-U.
Screening of the complete genome sequence from the yeast Saccharomyces cerevisiae has enabled us to compile a complete list of the genes encoding cytoplasmic ribosomal proteins in this organism. Putative ribosomal protein genes were selected primarily on the basis of the sequence similarity of their products with ribosomal proteins from other eukaryotic organisms, in particular the rat. These genes were subsequently screened for typical yeast rp-gene characteristics, viz. (1) a high codon adaptation index; (2) their promoter structure and (3) their responses to changes in growth conditions. The yeast genome appears to carry 78 different genes, of which 59 are duplicated, encoding 32 different small-subunit and 46 large-subunit proteins. A new nomenclature for these ribosomal proteins is proposed.
对酿酒酵母完整基因组序列的筛选，使我们能够编制出该生物体中编码细胞质核糖体蛋白的基因的完整清单。推定的核糖体蛋白基因主要是根据其产物与其他真核生物（特别是大鼠）的核糖体蛋白的序列相似性来选择的。随后对这些基因进行典型酵母核糖体蛋白基因特征的筛选，即：（1）高密码子适应指数；（2）它们的启动子结构；（3）它们对生长条件变化的反应。酵母基因组似乎携带78个不同的基因，其中59个是重复的，编码32种不同的小亚基蛋白和46种大亚基蛋白。本文提出了这些核糖体蛋白的新命名法。
