Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, 0028, South Africa.
Curr Genet. 2022 Aug;68(3-4):505-514. doi: 10.1007/s00294-022-01235-1. Epub 2022 Mar 22.
Resistance to the antibiotic Cycloheximide has been reported for a number of fungal taxa. In particular, some yeasts are known to be highly resistant to this antibiotic. Early research showed that this resulted from a transition mutation in one of the 60S ribosomal protein genes. In addition to the yeasts, most genera and species in the Ophiostomatales are highly resistant to this antibiotic, which is widely used to selectively isolate these fungi. Whole-genome sequences are now available for numerous members of the Ophiostomatales providing an opportunity to determine whether the mechanism of resistance in these fungi is the same as that reported for yeast genera such as Kluyveromyces. We examined all the available genomes for the Ophiostomatales and discovered that a transition mutation in the gene coding for ribosomal protein eL42, which results in the substitution of the amino acid Proline to Glutamine, likely confers resistance to this antibiotic. This change across all genera in the Ophiostomatales suggests that the mutation arose early in the evolution of these fungi.
已有报道称，许多真菌类群对环丝氨酸抗生素具有抗性。特别是，一些酵母被认为对这种抗生素具有高度抗性。早期的研究表明，这是由于 60S 核糖体蛋白基因之一发生了转换突变。除了酵母，小煤炱目中的大多数属和种对这种抗生素也具有高度抗性，这种抗生素被广泛用于选择性分离这些真菌。现在有许多小煤炱目的成员的全基因组序列，这为确定这些真菌的抗性机制是否与酵母属如克鲁维酵母等报告的机制相同提供了机会。我们检查了小煤炱目所有可用的基因组，发现核糖体蛋白 eL42 基因编码的转换突变导致脯氨酸被谷氨酰胺取代，可能赋予了对这种抗生素的抗性。这种突变在小煤炱目中的所有属中都存在，表明这种突变是在这些真菌的早期进化中产生的。
