Wachsmuth E D
Histochemistry. 1976 Aug 12;48(2):101-9. doi: 10.1007/BF00494548.
Sections from human jejunum were stained histochemically for aminopeptidase and alkaline phosphatase and the aldolase isozymes were detected with the mixed aggregation immuno-cytochemical technique. All enzyme concentrations increased from the bottom to the upper part of the crypt. The concentration of aldolase-A per cell was the same in the upper part of the crypt and the villus, whereas the concentration of the other three enzymes was still higher. Therefore, high amounts of aldolase-B, aminopeptidase and alkaline phosphatase are present in cells highly active in absorption in a fashion similar to that found in the proximal tubule cells of kidney. The relatively undifferentiated cells of the crypts contained both aldolase-A and aldolase-B. Alkaline phosphatase gains its full activity later than aminopeptidase. The synthesis of microvillar membrane enzymes comes to an end earlier than that of the cytosol enzymes.
对人空肠切片进行氨基肽酶和碱性磷酸酶的组织化学染色，并用混合聚集免疫细胞化学技术检测醛缩酶同工酶。所有酶浓度从隐窝底部到上部均升高。隐窝上部和绒毛中每个细胞的醛缩酶 -A 浓度相同，而其他三种酶的浓度仍然更高。因此，在吸收活跃的细胞中存在大量的醛缩酶 -B、氨基肽酶和碱性磷酸酶，其方式类似于在肾近端小管细胞中发现的情况。隐窝中相对未分化的细胞同时含有醛缩酶 -A 和醛缩酶 -B。碱性磷酸酶比氨基肽酶更晚获得其全部活性。微绒毛膜酶的合成比胞质溶胶酶更早结束。
