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用于检测类囊体膜中活性氧的双(荧光和自旋)传感器。

Double (fluorescent and spin) sensors for detection of reactive oxygen species in the thylakoid membrane.

作者信息

Kálai T, Hideg E, Vass I, Hideg K

机构信息

Institute of Organic and Medicinal Chemistry, University of Pécs, Hungary.

出版信息

Free Radic Biol Med. 1998 Mar 1;24(4):649-52. doi: 10.1016/s0891-5849(97)00339-0.

DOI:10.1016/s0891-5849(97)00339-0
PMID:9559877
Abstract

A series of dansylated sterically hindered amines designed to trapping reactive oxygen species, were synthesized. Compounds were tested in isolated thylakoid membranes subjected to photoinhibition by excess photosynthetically active radiation (400-700 nm). DanePy showed good selectivity for singlet oxygen and the formation of nitroxide was detected by appearance of ESR signal and quenching fluorescence.

摘要

合成了一系列旨在捕获活性氧的丹磺酰化位阻胺。在受到过量光合有效辐射(400 - 700 nm)光抑制的分离类囊体膜中对化合物进行了测试。DanePy对单线态氧表现出良好的选择性,通过电子顺磁共振信号的出现和荧光猝灭检测到了氮氧化物的形成。

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