纳米 FN10：一种用于多光子单线态氧成像的高对比度荧光纳米探针。

Departamento de Química Orgánica y Fisicoquímica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago 8330015, Chile.

Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08017 Barcelona, Spain.

Sensors (Basel). 2023 May 9;23(10):4603. doi: 10.3390/s23104603.

An "off-on" fluorescent nanoprobe for near-infrared multiphoton imaging of singlet oxygen has been developed. The nanoprobe comprises a naphthoxazole fluorescent unit and a singlet-oxygen-sensitive furan derivative attached to the surface of mesoporous silica nanoparticles. In solution, the fluorescence of the nanoprobe increases upon reaction with singlet oxygen both under one- and multiphoton excitation, with fluorescence enhancements up to 180-fold. The nanoprobe can be readily internalized by macrophage cells and is capable of imaging intracellular singlet oxygen under multiphoton excitation.

一种用于单线态氧的近红外多光子成像的“开-关”荧光纳米探针已经被开发出来。该纳米探针由萘恶唑荧光单元和附着在介孔硅纳米粒子表面的单线态氧敏感呋喃衍生物组成。在溶液中，纳米探针在单光子和多光子激发下与单线态氧反应时，荧光会增强，荧光增强可达 180 倍。该纳米探针可以很容易地被巨噬细胞内化，并能够在多光子激发下对细胞内的单线态氧进行成像。